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Molecular cytogenetic findings in a three-way novel variant of t(1;8;21)(p35;q22;q22): a unique relocation of the AML1/ETO fusion gene 1p35 in AML-M2.
Ahmad, Firoz; Kokate, Prajakta; Chheda, Pratiksha; Dalvi, Rupa; Das, Bibhu Ranjan; Mandava, Swarna.
Afiliación
  • Ahmad F; Research and Development, SRL Ranbaxy Ltd., MIDC, Andheri (E), Mumbai-400093, India.
Cancer Genet Cytogenet ; 180(2): 153-7, 2008 Jan 15.
Article en En | MEDLINE | ID: mdl-18206543
Acute myeloid leukemia (AML) is a malignant neoplasm of hematopoietic stem cells characterized by an abnormal proliferation of myeloid precursors, a reduced rate of apoptosis, and an arrest in cellular differentiation. The present report deals with the results of hematologic, immunophenotypic, cytogenetic, fluorescence in situ hybridization (FISH), and molecular analyses of a 53-year-old female patient diagnosed with AML-M2. Cytogenetic and FISH analysis revealed a complex translocation involving three chromosomes showing t(1;8;21)(p35;q22;q22). The observation of breakpoints at 8q22 and 21q22 suggests a rearrangement of the ETO and AML1 genes, respectively. Using a dual-color FISH test with ETO and AML1 probes, an AML1/ETO fusion signal on the derivative 1p35 instead of der(8) was demonstrated. To the best of our knowledge, this is the first report about the relocation of the AML1/ETO fusion gene to the 1p35 rather than der(8), suggesting the presence of a novel variant of t(8;21)(q22;q22) in the observed patient.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Translocación Genética / Cromosomas Humanos Par 1 / Cromosomas Humanos Par 8 / Cromosomas Humanos Par 21 / Leucemia Mieloide Aguda / Proteínas de Fusión Oncogénica / Subunidad alfa 2 del Factor de Unión al Sitio Principal Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Middle aged Idioma: En Revista: Cancer Genet Cytogenet Año: 2008 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Translocación Genética / Cromosomas Humanos Par 1 / Cromosomas Humanos Par 8 / Cromosomas Humanos Par 21 / Leucemia Mieloide Aguda / Proteínas de Fusión Oncogénica / Subunidad alfa 2 del Factor de Unión al Sitio Principal Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Middle aged Idioma: En Revista: Cancer Genet Cytogenet Año: 2008 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos