Presence and subcellular localizations of surfactant proteins A and D in human spermatozoa.
Fertil Steril
; 90(5): 1904-9, 2008 Nov.
Article
en En
| MEDLINE
| ID: mdl-18191856
OBJECTIVE: To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa and surfactant protein-D (SP-D); and molecular weight 43 kDa in human spermatozoa. DESIGN: Prospective, research study. SETTING: Two universities in Turkey. PATIENT(S): Semen specimens (n = 10) were obtained from normozoospermic donors. INTERVENTION(S): Human sperm were exposed to an anti-human SP-A polyclonal antibody, and monoclonal antibody, to human SP-D protein. MAIN OUTCOME MEASURE(S): Presence of SP-A and SP-D proteins in human beings. RESULT(S): Indirect immunofluorescence assays of human sperm indicated the presence of SP-A in the mid piece, the tail, and sometimes at the equatorial region of spermatozoa. A brilliant green light detected SP-D in the tails and acrosome of some sperm. The anti-SP-A antibody detected a single band corresponding to the molecular weight values of 34 kDa in spermatozoa, whereas no band was observed in the negative control. The anti-SP-D antibody showed the expected band at 43 kDa in spermatozoa. CONCLUSION(S): This is the first report and a novel finding of the presence of surfactant glycoproteins on human spermatozoa.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Proteína A Asociada a Surfactante Pulmonar
/
Proteína D Asociada a Surfactante Pulmonar
Tipo de estudio:
Clinical_trials
/
Observational_studies
/
Risk_factors_studies
Límite:
Humans
/
Male
País/Región como asunto:
Asia
Idioma:
En
Revista:
Fertil Steril
Año:
2008
Tipo del documento:
Article
País de afiliación:
Turquía
Pais de publicación:
Estados Unidos