Cell stiffening in response to external stress is correlated to actin recruitment.
Biophys J
; 94(7): 2906-13, 2008 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-18178644
We designed a micromanipulation device that allows the local application of a constant force on living cells, and the measurement of their stiffness. The force is applied through an Arg-Gly-Asp-coated bead adhering on the cell and trapped in optical tweezers controlled by a feedback loop. Epifluorescence observations of green fluorescent protein-actin in the cells are made during force application. We observe a stiffening of cells submitted to a constant force within a few minutes, coupled to actin recruitment both at the bead-cell contact and up to several micrometers from the stress application zone. Moreover, kinetics of stiffening and actin recruitment exhibit a strong correlation. This work presents the first quantification of the dynamics of cell mechanical reinforcement under stress, which is a novel insight into the elucidation of the more general phenomenon of cell adaptation to stress.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Adhesión Celular
/
Actinas
/
Mioblastos
/
Mecanotransducción Celular
/
Células Epiteliales
/
Pinzas Ópticas
/
Micromanipulación
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biophys J
Año:
2008
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos