Active immunization against macrophage migration inhibitory factor using a novel DNA vaccine prevents ovariectomy-induced bone loss in mice.
Vaccine
; 26(6): 829-36, 2008 Feb 06.
Article
en En
| MEDLINE
| ID: mdl-18178295
Previous studies have demonstrated that mice deficient in the macrophage migration inhibitory factor (MIF) gene are protected from ovariectomy (OVX)-induced bone loss. We developed a novel MIF-deoxyribonucleic acid (DNA) vaccine by introducing oligonucleotides encoding a helper T epitope into the cDNA sequence of murine MIF. Mice given the MIF-DNA vaccine produced high titers of autoantibody against MIF, and were protected from OVX-induced bone loss. Our results further support the hypothesis that MIF is involved in the pathomechanism of OVX-induced bone loss, and also show that active immunization against MIF using a DNA vaccine may be useful for the prophylactic treatment of postmenopausal osteoporosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoporosis
/
Factores Inhibidores de la Migración de Macrófagos
/
Vacunación
/
Vacunas de ADN
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Vaccine
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Países Bajos