Thiamine limitation determines the transition from aerobic to fermentative-like metabolism in Rhizobium etli CE3.
FEMS Microbiol Lett
; 279(1): 48-55, 2008 Feb.
Article
en En
| MEDLINE
| ID: mdl-18177306
Both thiamine and biotin when added to minimal medium subcultures reversed the fermentative-like metabolism exhibited by Rhizobium etli CE3. Thiamine auxotrophs lacking thiCOGE genes were used to investigate the role of thiamine in this medium. A thiC1169::miniTn5lacZ1 thiamine auxotroph subjected to the above subcultures resulted in growth arrest, reduced pyruvate-dehydrogenase activity, and a smaller amount of poly-beta-hydroxybutyrate compared with the CE3 strain. Moreover, thiC and thiEb genes were overexpressed as result of thiamine limitation. The absence of classical thi genes suggests that thiamine is synthesized with low efficiency by an alternative pathway. Low levels of thiamine cause the CE3 strain to exhibit a fermentative-like metabolism.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tiamina
/
Rhizobium etli
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2008
Tipo del documento:
Article
Pais de publicación:
Reino Unido