The LysR-type transcriptional regulator LeuO controls expression of several genes in Salmonella enterica serovar Typhi.
J Bacteriol
; 190(5): 1658-70, 2008 Mar.
Article
en En
| MEDLINE
| ID: mdl-18156266
LeuO is a LysR-type transcriptional regulator that has been implicated in the bacterial stringent response and in the virulence of Salmonella. A genomic analysis with Salmonella enterica serovar Typhi revealed that LeuO is a positive regulator of OmpS1, OmpS2, AssT, and STY3070. In contrast, LeuO down-regulated the expression of OmpX, Tpx, and STY1978. Transcriptional fusions supported the positive and negative LeuO regulation. Expression of ompS1, assT, and STY3070 was induced in an hns mutant, consistent with the notion that H-NS represses these genes; transcriptional activity was lower for tpx and STY1978 in an hns background, suggesting that this global regulatory protein has a positive effect. In contrast, ompS2 and ompX expression appeared to be H-NS independent. LeuO specifically bound to the 5' intergenic regions of ompS2, assT, STY3070, ompX, and tpx, while it was not observed to bind to the promoter region of STY1978, suggesting that LeuO regulates in direct and indirect ways. In this work, a novel set of genes belonging to the LeuO regulon are described; interestingly, these genes are involved in a variety of biological processes, suggesting that LeuO is a global regulator in Salmonella.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Salmonella typhi
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Proteínas de la Membrana Bacteriana Externa
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Regulación Bacteriana de la Expresión Génica
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Transactivadores
Idioma:
En
Revista:
J Bacteriol
Año:
2008
Tipo del documento:
Article
Pais de publicación:
Estados Unidos