Iron oxide labelling of human mesenchymal stem cells in collagen hydrogels for articular cartilage repair.
Biomaterials
; 29(10): 1473-83, 2008 Apr.
Article
en En
| MEDLINE
| ID: mdl-18155133
For the development of new therapeutical cell-based strategies for articular cartilage repair, a reliable cell monitoring technique is required to track the cells in vivo non-invasively and repeatedly. We present a systematic and detailed study on the performance and biological impact of a simple and efficient labelling protocol for human mesenchymal stem cells (hMSCs). Commercially available very small superparamagnetic iron oxide particles (VSOPs) were used as magnetic resonance (MR) contrast agent. Iron uptake via endocytosis was confirmed histologically with prussian blue staining and quantified by mass spectrometry. Compared with unlabelled cells, VSOP-labelling did neither influence the viability nor the proliferation potential of hMSCs. Furthermore, iron incorporation did not affect hMSCs in undergoing adipogenic, osteogenic or chondrogenic differentiation, as demonstrated histologically and by gene expression analyses. The efficiency of the labelling protocol was assessed with high-resolution MR imaging at 11.7T. VSOP-labelled hMSCs were visualised in a collagen type I hydrogel, which is in clinical use for matrix-based articular cartilage repair. The presence of VSOP-labelled hMSCs was indicated by distinct hypointense spots in the MR images, as a result of iron specific loss of signal intensity. In summary, this labelling technique has great potential to visualise hMSCs and track their migration after transplantation for articular cartilage repair with MR imaging.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Férricos
/
Cartílago Articular
/
Colágeno
/
Células Madre Mesenquimatosas
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
Biomaterials
Año:
2008
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Países Bajos