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Involvement of mature donor T cells in the NK cell reconstitution after haploidentical hematopoietic stem-cell transplantation.
Nguyen, S; Kuentz, M; Vernant, J-P; Dhedin, N; Bories, D; Debré, P; Vieillard, V.
Afiliación
  • Nguyen S; Laboratoire d'Immunologie Cellulaire et Tissulaire, Institut National de la Santé et de la Recherche Médicale U543, Hôpital Pitié-Salpêtrière, Paris, France.
Leukemia ; 22(2): 344-52, 2008 Feb.
Article en En | MEDLINE | ID: mdl-18033316
We previously demonstrated that natural killer (NK) cells generated after haploidentical stem-cell transplantation (SCT) are blocked at an immature state characterized by phenotypic features and impaired functioning and that this may affect transplantation outcome. We hypothesize that the absence of mature donor T cells in the graft may affect NK cell differentiation. NK cells from 21 transplant recipients who underwent either partial (pTCD; n=11) or extensive (eTCD; n=10) T-cell depletion were compared with NK cells from their healthy donors. We report that despite the strong graft-versus-host disease (GvHD) reaction, pTCD patients, with T cells present during SCT, had a better clinical outcome than patients with eTCD transplants. In addition, the frequency of CD3- CD56(bright) and NKG2A+ NK cells was much lower in pTCD than in eTCD patients after transplantation, and the level of cytotoxicity against primary haplo-mismatched blasts was significantly more pronounced after pTCD than eTCD transplants. These finding strongly suggest that mature donor T cells in the graft may play a key role in NK cell differentiation in vivo, after haploidentical SCT.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Células Asesinas Naturales / Linfocitos T / Depleción Linfocítica / Trasplante de Células Madre Hematopoyéticas Límite: Adolescent / Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Leukemia Asunto de la revista: HEMATOLOGIA / NEOPLASIAS Año: 2008 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Células Asesinas Naturales / Linfocitos T / Depleción Linfocítica / Trasplante de Células Madre Hematopoyéticas Límite: Adolescent / Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Leukemia Asunto de la revista: HEMATOLOGIA / NEOPLASIAS Año: 2008 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido