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Expression of p16 and cervical infection with high-risk human papillomavirus are not related to p53 activity in cervical intraepithelial neoplasia.
Bragança, J F; Sarian, L O; Pitta, D R; Maito, A B; Vassallo, J; Pignataro, F; Andrade, L A; Derchain, S.
Afiliación
  • Bragança JF; Departments of Obstetrics and Gynecology and Department of Pathology, Universidade Estadual de Campinas (Unicamp), Campinas, Brazil.
Int J Gynecol Cancer ; 18(5): 1060-4, 2008.
Article en En | MEDLINE | ID: mdl-18021213
The objective of the study was to investigate the expression of p53 and p16(INK4a) in cervical intraepithelial neoplasia (CIN) and their relation with disease severity and high-risk human papillomavirus (HR-HPV) status. A series of 125 women with previous positive Pap smear were selected for this cross-sectional study. All patients underwent gynecological examination, including colposcopy. Specimens for Pap smears, Hybrid Capture 2 (HC2) test, and pathologic analysis were obtained. After evaluation of CIN grade, immunohistochemical detection of proteins p53 and p16(INK4a) was performed on paraffin-embedded sections. The extent of immunoexpression of both proteins was analyzed in relation to CIN grade and HR-HPV status. CIN was graded as 1 in 21, 2 in 17, and 3 in 87 specimens. p16(INK4a) positivity (at least 5% of epithelial cells stained) was found in 99 of 125 cases (79.2%) and was significantly higher in high-grade lesions as compared to low-grade CIN (P < 0.001). The expression of p53 did not differ across histologic strata. Protein expression neither of p16(INK4a) nor of p53 correlated with HR-HPV status. Expression of p16(INK4a) was not related with that of p53. Our study gives further support to previous findings of strong association of p16(INK4a) immunostaining with severity of epithelial atypia, but this protein may not be considered a predictor of HR-HPV status determined with HC2. By contrast, immunoexpression of p53 was related neither to CIN grade nor to HR-HPV status.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Displasia del Cuello del Útero / Inhibidor p16 de la Quinasa Dependiente de Ciclina / Infecciones por Papillomavirus Tipo de estudio: Etiology_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adult / Female / Humans / Middle aged Idioma: En Revista: Int J Gynecol Cancer Asunto de la revista: GINECOLOGIA / NEOPLASIAS Año: 2008 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Buscar en Google
Añadir a Mi BVS
Imprimir
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Displasia del Cuello del Útero / Inhibidor p16 de la Quinasa Dependiente de Ciclina / Infecciones por Papillomavirus Tipo de estudio: Etiology_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adult / Female / Humans / Middle aged Idioma: En Revista: Int J Gynecol Cancer Asunto de la revista: GINECOLOGIA / NEOPLASIAS Año: 2008 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido