Kinetic analysis reveals potency of CD4+ CD25bright+ regulatory T-cells in kidney transplant patients.
Transpl Immunol
; 18(2): 159-65, 2007 Nov.
Article
en En
| MEDLINE
| ID: mdl-18005862
Donor-specific hyporesponsiveness as occurs after allogeneic kidney transplantation may be mediated by repression of effector cells by a specific subset of T-cells: the CD4(+) CD25(bright+) FoxP3(+) regulatory T-cells (Tregs). Here, we examined the suppressive capacity of Tregs isolated from the leukafereses product of 6 kidney transplant recipients, by reconstituting Tregs to responder T-cells at several time-points after initiation of proliferation. We show that Tregs derived from kidney transplant patients potently restrain proliferation to donor-antigens and 3rd party-antigens in classic reconstitution assays (i.e. addition of Tregs at the start of the co-incubation). However, when Tregs were added 5 days after initiation of proliferation, they were still capable of suppressing proliferation to donor-antigens (by 38%) but no longer to 3rd party-antigens. Thus, we conclude that the potency of Tregs to suppress reactivity to specific antigens should be determined by reconstitution to ongoing reactions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antígenos CD4
/
Trasplante de Riñón
/
Linfocitos T Reguladores
/
Subunidad alfa del Receptor de Interleucina-2
Límite:
Humans
/
Male
Idioma:
En
Revista:
Transpl Immunol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
TRANSPLANTE
Año:
2007
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Países Bajos