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Protection against Shiga toxin-producing Escherichia coli infection by transcutaneous immunization with Shiga toxin subunit B.
Zhu, C; Yu, J; Yang, Z; Davis, K; Rios, H; Wang, B; Glenn, G; Boedeker, E C.
Afiliación
  • Zhu C; Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
Clin Vaccine Immunol ; 15(2): 359-66, 2008 Feb.
Article en En | MEDLINE | ID: mdl-18003816
Enterohemorrhagic Escherichia coli (EHEC) strains are important human food-borne pathogens. EHEC strains elaborate potent Shiga toxins (Stx1, and/or Stx2) implicated in the development of hemorrhagic colitis (HC) or hemolytic-uremic syndrome (HUS). In this report, we evaluated the immunogenicity and protective efficacy of Stx1 subunit B (StxB1) administered by transcutaneous immunization (TCI). Three groups of Dutch Belted rabbits received patches containing StxB1, StxB1 in combination with Escherichia coli heat-labile enterotoxin (LT), or LT alone. An additional group of naïve rabbits served as controls. The protective efficacy following TCI with StxB1 was assessed by challenging rabbits with a virulent Stx1-producing strain, RDEC-H19A, capable of inducing HC and HUS in rabbits. Antibodies specific to StxB1 from serum and bile samples were determined by enzyme-linked immunosorbent assay and toxin neutralization test. Rabbits immunized with StxB1 demonstrated improved weight gain and reduced Stx-induced histopathology. Rabbits receiving StxB or StxB1/LT showed a significant increase in serum immunoglobulin G titers specific to StxB1 as well as toxin neutralization titers. These data demonstrated that the StxB delivered by TCI could induce significant systemic immune responses. Thus, Stx subunit B vaccine delivered by a patch for a high-risk population may be a practical approach to prevent (and/or reduce) Stx-induced pathology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Administración Cutánea / Toxina Shiga / Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica Límite: Animals Idioma: En Revista: Clin Vaccine Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2008 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Administración Cutánea / Toxina Shiga / Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica Límite: Animals Idioma: En Revista: Clin Vaccine Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2008 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos