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Effects of tobacco genetically modified to express protease inhibitor bovine spleen trypsin inhibitor on non-target soil organisms.
O'Callaghan, Maureen; Brownbridge, Michael; Stilwell, Wendy B; Gerard, Emily M; Burgess, Elisabeth P J; Barraclough, Emma I; Christeller, John T.
Afiliación
  • O'Callaghan M; Biocontrol, Biosecurity and Bioprocessing Section, AgResearch, Lincoln Research Centre, Private Bag 4749, Christchurch, New Zealand. maureen.ocallaghan@agresearch.co.nz
Environ Biosafety Res ; 6(3): 183-95, 2007.
Article en En | MEDLINE | ID: mdl-18001685
Effects of tobacco genetically modified to express the protease inhibitor bovine spleen trypsin inhibitor (BSTI) were examined in laboratory assays against three earthworm and one collembolan species. BSTI is a serine protease inhibitor that can bind to the digestive trypsins of insects feeding on modified plants, resulting in reduced growth and survival. Protease inhibitors are active against a broad range of insects, so may have a large impact on non-target organisms. Survival and fecundity of the collembolan Folsomia candida were unaffected by consumption of artificial diet containing BSTI-expressing tobacco leaf or powdered freeze-dried BSTI-expressing tobacco leaf that was added to soil. Similarly, mortality and growth of earthworms Aporrectodea caliginosa and Lumbricus rubellus did not differ significantly between soil augmented with BSTI-expressing tobacco leaves or unmodified control leaves. The redworm Eisenia fetida gained less weight when provided with BSTI-expressing leaves in one assay, but when the experiment was repeated, there was no significant difference between treatments. BSTI-expressing tobacco and unmodified control leaves decomposed at the same rate, indicating that the inhibitor had no effect on the overall function of the decomposer community of micro-flora and fauna in soil.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligoquetos / Artrópodos / Nicotiana / Inhibidores de Tripsina / Plantas Modificadas Genéticamente Límite: Animals Idioma: En Revista: Environ Biosafety Res Asunto de la revista: SAUDE AMBIENTAL Año: 2007 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Francia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligoquetos / Artrópodos / Nicotiana / Inhibidores de Tripsina / Plantas Modificadas Genéticamente Límite: Animals Idioma: En Revista: Environ Biosafety Res Asunto de la revista: SAUDE AMBIENTAL Año: 2007 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Francia