Simultaneous determination of active ingredients in ethnomedicine Gaultheria leucocarpa var. yunnanensis and its medicinal preparation by capillary electrophoresis with electrochemical detection.
J Chromatogr Sci
; 45(9): 610-5, 2007 Oct.
Article
en En
| MEDLINE
| ID: mdl-17988450
A simple and rapid capillary electrophoresis (CE) with electrcochemical detection (ED) method has been established for the simultaneous determination of seven active ingredients in the stems and roots of Gaultheria leucocarpa var. yunnanensis and its medicinal preparation, including (+)-catechin, rutin, gentisic acid, vallinic acid, salicylic acid, quercetin, and protocatechuic acid. The effects of working potential, pH, and concentration of running buffer, separation voltage, and injection time on CE-ED are systematically investigated. Under the optimum conditions, the seven analytes could be completely separated within 23 min in a borax running buffer (pH 8.7). A good linear relationship is obtained over three orders of magnitude with detection limits (signal-to-noise ratio=3) ranging from 5x10(-8) g/mL to 3x10(-7) g/mL for the analytes. The proposed method is successfully used in the analysis of real samples after a relatively simple extraction procedure, and the assay results are satisfactory.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Electroforesis Capilar
/
Preparaciones de Plantas
/
Gaultheria
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr Sci
Año:
2007
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Estados Unidos