A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells.

Zeerleder, Sacha; Zwart, Bas; te Velthuis, Henk; Manoe, Rishi; Bulder, Ingrid; Rensink, Irma; Aarden, Lucien A

Zeerleder, Sacha; Zwart, Bas; te Velthuis, Henk; Manoe, Rishi; Bulder, Ingrid; Rensink, Irma; Aarden, Lucien A.

Afiliación

Zeerleder S; Department of Immunopathology, Sanquin, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands.

FEBS Lett ; 581(28): 5382-8, 2007 Nov 27.

Article en En | MEDLINE | ID: mdl-17976375

RESUMEN

We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells.

Asunto(s)

Nucleosomas/enzimología; Serina Endopeptidasas/metabolismo; Activación Enzimática; Fibrinolisina/metabolismo; Humanos; Células Jurkat; Necrosis/enzimología; Necrosis/patología; Nucleosomas/efectos de los fármacos; Plasminógeno/metabolismo; Propidio; Inhibidores de Proteasas/farmacología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Serina Endopeptidasas / Nucleosomas Límite: Humans Idioma: En Revista: FEBS Lett Año: 2007 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido

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