Late-bolting transgenic Chinese cabbage obtained by RNA interference technique.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
; 33(5): 411-6, 2007 Oct.
Article
en En
| MEDLINE
| ID: mdl-17960044
LEAFY (LFY) gene plays an important role in determining plant flowering mainly by controlling the timing of phase transition. Constitutive under-expression of LFY in Arabidopsis resulted in the formation of a late-flowering and highly branching phenotype. In this paper, an RNAi approach was used in down-regulated LFY gene expression to delay Chinese cabbage (Brassica rapa L. ssp. pekinensis) bolting and flowering. The results show that transgenic plant has a later transition to the reproductive phase, and the transgenic plants have more branches, more leaves, but a lower height. Results of RQ-RT-PCR analysis show that LFY gene expression was greatly reduced in transgenic plants. These results suggest that inhibiting LFY gene expression by RNA interference can delay bolting in a cold-sensitive long-day (LD) condition. Late flowering of Chinese cabbage can be used as a good genetic resource for the breeding late-bolting Chinese cabbage.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Brassica
/
Plantas Modificadas Genéticamente
/
Interferencia de ARN
Idioma:
En
Revista:
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China