Validating a QTL region characterized by multiple haplotypes.
Theor Appl Genet
; 116(1): 87-94, 2007 Dec.
Article
en En
| MEDLINE
| ID: mdl-17938879
Validation of a quantitative trait locus (QTL) for outcrossing perennial plants is rarely reported due to complexity of plausible genetic models and reliance on field designs already available. Here, a particular marker-QTL haplotype exerted a large, positive effect on height for Pinus taeda and its origin could be traced to a founder, GP(3), in a three-generation QTL pedigree. To validate this QTL effect, we used an extended GP(3)-based pedigree. In the validation cross, each of the 46 offspring was clonally propagated from developing seeds using somatic embryogenesis technology. Subsequent analyses were conducted separately for seedlings and for other somatic emblings. For seedlings, the original QTL effect could not be fully validated. For somatic emblings, a strong negative QTL effect was detected in the validation cross; some evidence from another cross-supported the original positive QTL effect. From this part of the analysis, three distinct marker-QTL haplotypes at a single locus could be inferred. Validating QTL haplotypes in readily available field tests was feasible despite the genetic model complexity inherent to outcrossing long-lived perennials.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Haplotipos
/
Sitios de Carácter Cuantitativo
/
Pinus taeda
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Theor Appl Genet
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Alemania