Effects of intraocular irrigation with melphalan on rabbit retinas during vitrectomy.
Graefes Arch Clin Exp Ophthalmol
; 246(4): 501-8, 2008 Apr.
Article
en En
| MEDLINE
| ID: mdl-17934752
PURPOSE: To investigate the toxic effects of perfusion of intravitreal melphalan during vitrectomy on the rabbit retina. METHODS: We performed electoretinography (ERG) in 18 eyes of 18 healthy albino rabbits before and after intraocular melphalan perfusion at concentrations of 5-, 10-, and 20-microg/ml during pars plana vitrectomy. Fellow eyes that underwent vitrectomy without melphalan served as controls. The histopathologic retinal changes were observed in both eyes of two rabbits from each group. RESULTS: In the 5-microg/ml perfusion group, the ERGs and histology showed no substantial changes compared with control fellow eyes during 28 days postoperatively. In the 10- and 20-microg/ml groups, the mean a-wave amplitude decreased to 52% and 31% respectively of the fellow eye; the mean b-wave amplitude decreased to 52% and 19% respectively. However, the peak implicit time of the a- and b-waves did not significantly differ in the 10- and 20-microg/ml groups during 28 days postoperatively. Histologic sections showed necrosis of the inner nuclear layer and thinning of the outer nuclear layer in the 10-microg/ml group. Loss of the outer nuclear layer and the photoreceptor layer and necrosis of the inner nuclear layer were observed in the 20-microg/ml group. CONCLUSION: The intravitreal 5-microg/ml melphalan perfusion during vitrectomy appears to be nontoxic to the retina. This therapeutic modality might be a potential treatment for retinoblastoma with vitreous seeding.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Retina
/
Vitrectomía
/
Antineoplásicos Alquilantes
/
Melfalán
Límite:
Animals
Idioma:
En
Revista:
Graefes Arch Clin Exp Ophthalmol
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Alemania