Cocrystallizing natural RNA with its unnatural mirror image: biochemical and preliminary X-ray diffraction analysis of a 5S rRNA A-helix racemate.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 63(Pt 10): 839-43, 2007 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-17909284
Chemically synthesized RNAs with the unnatural L-configuration possess enhanced in vivo stability and nuclease resistance, which is a highly desirable property for pharmacological applications. For a structural comparison, both L- and D-RNA oligonucleotides of a shortened Thermus flavus 5S rRNA A-helix were chemically synthesized. The enantiomeric RNA duplexes were stochiometrically cocrystallized as a racemate, which enabled analysis of the D- and L-RNA enantiomers in the same crystals. In addition to a biochemical investigation, diffraction data were collected to 3.0 A resolution using synchrotron radiation. The crystals belonged to space group P3(1)21, with unit-cell parameters a = b = 35.59, c = 135.30 A, gamma = 120 degrees and two molecules per asymmetric unit.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Difracción de Rayos X
/
ARN Ribosómico 5S
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Año:
2007
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Reino Unido