[Initial study on discrimination of oral microorganisms with the metabonomics technique].
Hua Xi Kou Qiang Yi Xue Za Zhi
; 25(4): 342-4, 2007 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-17896487
OBJECTIVE: To evaluate the feasibility of employing metabonomics method in identification of oral pathogenic bacteria. METHODS: The Streptococcus mutans ATCC25175 and Actinomyces viscosus ATCC15987 were respectively inoculated in same certain culture medium. The growth curves of the inoculated bacteria were drown by turbidimetry. The culture solutions in four different growth phases of the both bacteria were used to test with the 1H-Nuclear magnetic resonance (1H-NMR) spectroscopy respectively. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA). RESULTS: The PCA showed the obvious clustering phenomena and the points of two group data stayed differentially together by two clusters. Therefore, the NMR-based metabonomics profiles can discriminate the two different kind of bacteria. CONCLUSION: The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral pathogenic bacteria.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Streptococcus mutans
/
Metabolómica
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
Hua Xi Kou Qiang Yi Xue Za Zhi
Asunto de la revista:
ODONTOLOGIA
Año:
2007
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China