Localization of interchromatin granule cluster and Cajal body components in oocyte nuclear bodies of the hemipterans.
Tissue Cell
; 39(5): 353-64, 2007 Oct.
Article
en En
| MEDLINE
| ID: mdl-17889915
An oocyte nucleus contains different extrachromosomal nuclear domains collectively called nuclear bodies (NBs). In the present work we revealed, using immunogold labeling electron microscopy, some marker components of interchromatin granule clusters (IGCs) and Cajal bodies (CBs) in morphologically heterogeneous oocyte NBs studied in three hemipteran species: Notostira elongata, Capsodes gothicus (Miridae) and Velia caprai (Veliidae). Both IGC and CB counterparts were revealed in oocyte nuclei of the studied species but morphological and biochemical criteria were found to be not sufficient to determine carefully the define type of oocyte NBs. We found that the molecular markers of the CBs (coilin and non-phosphorylated RNA polymerase II) and IGCs (SC35 protein) may be localized in the same NB. Anti-SC35 antibody may decorate not only a granular material representing "true" interchromatin granules but also masks some fibrillar parts of complex NBs. Our first observations on the hemipteran oocyte NBs confirm the high complexity and heterogeneity of insect oocyte IGCs and CBs in comparison with those in mammalian somatic cells and amphibian oocytes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oocitos
/
Cromatina
/
Núcleo Celular
/
Cuerpos Enrollados
/
Gránulos Citoplasmáticos
/
Hemípteros
Límite:
Animals
Idioma:
En
Revista:
Tissue Cell
Año:
2007
Tipo del documento:
Article
País de afiliación:
Rusia
Pais de publicación:
Reino Unido