Molecular evidence for the ubiquitous presence of Legionella species in Dutch tap water installations.
J Water Health
; 5(3): 375-83, 2007 Sep.
Article
en En
| MEDLINE
| ID: mdl-17878552
Our aim was to investigate the occurrence and identity of Legionella spp. in Dutch tap water installations using culture, real-time PCR and sequence analysis. The PCR assays used were a 16S rRNA gene based PCR with both a Legionella species specific probe and a L. pneumophila specific probe and a L. pneumophila-specific PCR based on the sequence of the mip gene. A total of 357 water samples from 250 locations in The Netherlands was investigated. The detection rates of Legionella spp. were 2,2% (8 of 357) by culture, and 87,1% (311 of 357) by PCR. The majority of samples was found to contain Legionella species other than L. pneumophila. These comprised of Legionella Like Amoebal Pathogens (LLAPs), L. busanensis, L. worsliensis and others. Fourteen (3,9%) samples were positive for L. pneumophila by either culture, 16S rRNA based PCR and/or mip based PCR. It is apparent from this study that Legionella spp. DNA is ubiquitous in Dutch potable water samples. Our findings further suggest that LLAPs and viable but nonculturable (VBNC) Legionella represent a large proportion of the population in man-made environments.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Contaminantes del Agua
/
Abastecimiento de Agua
/
Legionella
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Water Health
Asunto de la revista:
SAUDE AMBIENTAL
Año:
2007
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Reino Unido