Time gap between oocyst shedding and antibody responses in mice infected with Cryptosporidium parvum.
Korean J Parasitol
; 45(3): 225-8, 2007 Sep.
Article
en En
| MEDLINE
| ID: mdl-17876169
We observed the time gap between oocyst shedding and antibody responses in mice (3-week-old C57BL/6J females) infected with Cryptosporidium parvum. Oocyst shedding was verified by modified acid-fast staining. The individually collected mouse sera were assessed for C. parvum IgM and IgG antibodies by enzyme-linked immunosorbent assay from 5 to 25 weeks after infection. The results showed that C. parvum oocysts were shed from day 5 to 51 post-infection (PI). The IgM antibody titers to C. parvum peaked at week 5 PI, whereas the IgG antibody titers achieved maximum levels at week 25 PI. The results revealed that IgM responses to C. parvum infection occurred during the early stage of infection and overlapped with the oocyst shedding period, whereas IgG responses occurred during the late stage and was not correlated with oocyst shedding. Hence, IgM antibody detection may prove helpful for the diagnosis of acute cryptosporidiosis, and IgG antibody detection may prove effective for the detection of past infection and endemicity.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Anticuerpos Antiprotozoarios
/
Cryptosporidium parvum
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Criptosporidiosis
Límite:
Animals
Idioma:
En
Revista:
Korean J Parasitol
Año:
2007
Tipo del documento:
Article
Pais de publicación:
Corea del Sur