Liquid chromatographic separation of the enantiomers of metoprolol and its alpha-hydroxy metabolite on Chiralcel OD for determination in plasma and urine.
J Chromatogr
; 553(1-2): 391-7, 1991 Aug 16.
Article
en En
| MEDLINE
| ID: mdl-1787165
The two enantiomers of metoprolol and the four enantiomeric forms of alpha-hydroxymetoprolol were separated by liquid chromatography on a Chiralcel OD column containing a cellulose tris(3,5-dimethyl-phenylcarbamate) chiral stationary phase. The column efficiency was strongly dependent on the flow-rate and the enantioselectivity was influenced by temperature. Of utmost importance for the chiral separation was the water content of the mobile organic phase. The separation system was used for the separation and determination of the enantiomers in plasma and urine samples. The metoprolol enantiomers could be determined by fluorescence down to 10 nmol/l of each in plasma with a relative standard deviation of less than 15%.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Metoprolol
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Chromatogr
Año:
1991
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Países Bajos