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Effect of prebiotic supplementation on a probiotic bacteria mixture: comparison between a rat model and clinical trials.
Tiihonen, Kirsti; Suomalainen, Tarja; Tynkkynen, Soile; Rautonen, Nina.
Afiliación
  • Tiihonen K; Danisco Finland Oy, Sokeritehtaantie 20, FIN-02460 Kantvik, Finland. kirsti.tiihonen@danisco.com
Br J Nutr ; 99(4): 826-31, 2008 Apr.
Article en En | MEDLINE | ID: mdl-17868490
The effects of a probiotic mixture (PRO), supplemented with either galacto-oligosaccharide (GOS) or polydextrose (PDX), on cell numbers of lactic acid bacteria (LAB) and bifidobacteria (BIF) were studied in conventional rats and healthy human subjects. In rats the baseline BIF cell numbers were below the detection limit and were increased by the 2-week GOSPRO intervention. In contrast baseline LAB numbers in rats were high and not affected by the treatments. The human study consisted of two independent but concurrent trials; both started with PRO followed by GOSPRO or PDXPRO periods. In the human subjects variation in numbers of BIF and LAB were high. The GOSPRO group exhibited high counts of faecal LAB and BIF at the start and showed little or no effects of the interventions. In contrast, the PDX group had low faecal LAB and BIF numbers at the start and clearly increased cell numbers of BIF after the PDXPRO period, and LAB after the PRO and PDXPRO period, compared with the run-in period. We propose here that responses to pro- and prebiotics are dependent on baseline numbers of LAB and/or BIF, and that the conventional rat model does not predict well the treatment responses in humans. The survival of PRO was presumably enhanced by the use of prebiotic supplementation and advocates the use of particular combinations of pro- and prebiotics.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligosacáridos / Bifidobacterium / Probióticos / Glucanos / Intestinos / Lactobacillus Tipo de estudio: Clinical_trials / Prognostic_studies Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Br J Nutr Año: 2008 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligosacáridos / Bifidobacterium / Probióticos / Glucanos / Intestinos / Lactobacillus Tipo de estudio: Clinical_trials / Prognostic_studies Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Br J Nutr Año: 2008 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Reino Unido