Activation of T cells by cross-linking Qa-2, the ped gene product, requires Fyn.
Am J Reprod Immunol
; 58(4): 315-24, 2007 Oct.
Article
en En
| MEDLINE
| ID: mdl-17845201
PROBLEM: Qa-2, the product of the Ped (preimplantation development) gene, regulates the rate of cell division of preimplantation mouse embryos by an unknown mechanism. Due to the limited availability of preimplantation embryos, T cells were used as a model system to assess the possible roles of Fyn and Lck, and two downstream effectors, PI-3 kinase and Akt, in Qa-2 induced cell proliferation. METHOD OF STUDY: Resting T cells were stimulated to proliferate by treating with mouse anti-Qa-2 antibody, cross-linking with anti-mouse immunoglobulin, and adding PMA. The effects of kinase inhibitors on this proliferation were studied. Co-immunoprecipitates of T-cell lysates were analyzed for possible associations between Qa-2 and Fyn or Lck. Fyn knockout mice (Fyn-/-) were used to determine whether Fyn is required for T-cell activation induced by cross-linking Qa-2. RESULTS: An inhibitor of Src family kinases and inhibitors of PI-3 kinase and Akt suppressed proliferation of resting T cells induced by cross-linking Qa-2. Fyn, but not Lck, co-immunoprecipitated with Qa-2. Fyn-/- T cells failed to proliferate in response to Qa-2 cross-linking. CONCLUSION: Fyn, PI-3 kinase, and Akt are required for the activation of T cells by cross-linking Qa-2.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Activación de Linfocitos
/
Linfocitos T
/
Antígenos de Histocompatibilidad Clase I
/
Transducción de Señal
/
Proliferación Celular
/
Proteínas Proto-Oncogénicas c-fyn
Idioma:
En
Revista:
Am J Reprod Immunol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Dinamarca