Relatedness between Streptococcus pneumoniae and viridans streptococci: transfer of penicillin resistance determinants and immunological similarities of penicillin-binding proteins.
FEMS Microbiol Lett
; 69(1): 35-42, 1991 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-1783280
The occurrence of highly variable penicillin-binding proteins (PBPs) in penicillin-resistant Streptococcus pneumoniae suggested that transfer of homologous genes from related species may be involved in resistance development. Antiserum and monoclonal antibodies raised against PBPs 1a and 2b from the susceptible S. pneumoniae R6 strain were used to identify related PBPs in 41 S. mitis, S. sanguis I and S. sanguis II strains mostly isolated in South Africa with MIC values ranging from less than 0.15 to 16 mg/ml. Furthermore, the possibility of genetic exchange was examined with 30 penicillin-resistant strains of this collection (MIC greater than 0.06 mg/ml) as donors using S. pneumoniae R6 as recipient in transformation experiments. The majority of S. mitis and S. sanguis II strains but none of the S. sanguis I strains could transform penicillin resistance genes into S. pneumoniae R6. All positive donor strains and all susceptible isolates of S. mitis and S. sanguis II strains contained PBPs which cross-reacted with the anti-PBP 1a and/or anti-PBP 2b antibodies. On the other hand, only five of the 14 S. sanguis I strains contained a PBP that reacted with one of the antibodies. This strongly suggested the presence of genes homologous to the pneumococcal PBP 1a and 2b genes in viridans streptococci, and documents that penicillin resistance determinants can be transformed from viridans streptococci into the pneumococcus.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Streptococcus
/
Streptococcus pneumoniae
/
Proteínas Bacterianas
/
Transformación Genética
/
Resistencia a las Penicilinas
/
Muramoilpentapéptido Carboxipeptidasa
/
Proteínas Portadoras
/
Peptidil Transferasas
/
Hexosiltransferasas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
1991
Tipo del documento:
Article
Pais de publicación:
Reino Unido