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Basal, but not overload-induced, myonuclear addition is attenuated by NG-nitro-L-arginine methyl ester (L-NAME) administration.
Gordon, Scott E; Westerkamp, Christopher M; Savage, Kathleen J; Hickner, Robert C; George, Sarah C; Fick, Christopher A; McCormick, Kathleen M.
Afiliación
  • Gordon SE; Human Performance Laboratory, Department of Exercise and Sport Science, East Carolina University, Greenville, NC 27858, USA. gordonsc@ecu.edu
Can J Physiol Pharmacol ; 85(6): 646-51, 2007 Jun.
Article en En | MEDLINE | ID: mdl-17823627
The purpose of this study was to examine the effect of blocking nitric oxide synthase (NOS) activity via NG-nitro-L-arginine methyl ester (L-NAME) on myonuclear addition in skeletal muscle under basal and overloaded conditions. Female Sprague-Dawley rats (approx. 220 g) were placed into 1 of the following 4 groups (n = 7-9/group): 7-day skeletal muscle overload (O), sham operation (S), skeletal muscle overload with L-NAME treatment (OLN), and sham operation with L-NAME treatment (SLN). Plantaris muscles were overloaded via bilateral surgical ablation of the gastrocnemius muscles and L-NAME (0.75 mg/mL) was administered in the animals' daily drinking water starting 2 days prior to surgery and continued until sacrifice. Myonuclear addition was assessed as subsarcolemmal incorporation of nuclei labeled with 5-bromo-2'-deoxyuridine (approx. 25 mg.(kg body mass)-1.day-1) delivered via osmotic pump during the overload period. As expected, muscle wet mass, total protein content, fiber cross-sectional area, and myonuclear addition were significantly higher (p
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Celular / Músculo Esquelético / NG-Nitroarginina Metil Éster / Células Satélite del Músculo Esquelético Límite: Animals Idioma: En Revista: Can J Physiol Pharmacol Año: 2007 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Canadá
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Celular / Músculo Esquelético / NG-Nitroarginina Metil Éster / Células Satélite del Músculo Esquelético Límite: Animals Idioma: En Revista: Can J Physiol Pharmacol Año: 2007 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Canadá