Subcellular localization of glutamate dehydrogenases and alanine aminotransferase in epimastigotes of Trypanosoma cruzi.
FEMS Microbiol Lett
; 67(2): 131-5, 1991 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-1778428
The subcellular localization of NAD- and NADP-linked glutamate dehydrogenases (GDH-NAD and GDH-NADP), alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) in epimastigotes of Trypanosoma cruzi was studied by digitonin extraction from whole cells, subcellular fractionation by differential centrifugation and isopycnic ultracentrifugation. All enzymes presented both a cytosolic and a mitochondrial form; in addition, GDH-NADP seems to have a third, still undefined, localization. The results are compatible with the existence of two pathways for the production of L-alanine linked to the reoxidation of glycolytic NADH, one operative in the mitochondrion and the other in the cytosol, and perhaps responsible for the existence of the two alanine pools detected by 13C-nuclear magnetic resonance (B. Frydman et al., Eur. J. Biochem. 192 (1990) 363-368).
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aspartato Aminotransferasas
/
Trypanosoma cruzi
/
Alanina Transaminasa
/
Glutamato Deshidrogenasa
Límite:
Animals
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
1991
Tipo del documento:
Article
País de afiliación:
Argentina
Pais de publicación:
Reino Unido