[Culture of chondrocytes using nano-hydroxyapatite/collagen in vitro].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
; 32(4): 641-5, 2007 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-17767057
OBJECTIVE: To evaluate the value of the nano-hydroxyapatite/ collagen (nHAC) as a scaffold for cartilage tissue engineering. METHODS: The normal cartilage from patients after total hip arthroplasty of osteoarthritis was selected, and then chondrocytes were digested, separated, and amplificate in vitro. The chondrocytes were seeded onto the nHAC scaffold and were cultured in a 3-dimensional environment for 5 and 10 days. The effects of the composite scaffold on the cell adhesion, proliferation, morphological changes, and synthesis of the extracellular matrix were observed by scanning electronic microscopy and immunohistochemistry. RESULTS: The chondrocytes could adhere to the surface of the scaffolds, proliferate, and migrate into the scaffolds. They maintained round and could secrete extracellular matrix on the porous scaffold. CONCLUSION: The nHAC can be used as the cartilage cell carrier.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colágeno
/
Durapatita
/
Condrocitos
/
Ingeniería de Tejidos
/
Andamios del Tejido
Límite:
Humans
Idioma:
Zh
Revista:
Zhong Nan Da Xue Xue Bao Yi Xue Ban
Asunto de la revista:
MEDICINA
Año:
2007
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China