Inorganic polyphosphate: a possible stimulant of bone formation.
J Dent Res
; 86(9): 893-7, 2007 Sep.
Article
en En
| MEDLINE
| ID: mdl-17720862
Inorganic polyphosphates [Poly(P)] are often distributed in osteoblasts. We undertook the present study to verify the hypothesis that Poly(P) stimulates osteoblasts and facilitates bone formation. The osteoblast-like cell line MC 3T3-E1 was cultured with Poly(P), and gene expression and potential mineralization were evaluated by reverse-transcription polymerase chain-reaction. Alkaline phosphatase activity, von Kossa staining, and resorption pit formation analyses were also determined. The potential role of Poly(P) in bone formation was assessed in a rat alveolar bone regeneration model. Poly(P) induced osteopontin, osteocalcin, collagen 1alpha, and osteoprotegerin expression and increased alkaline phosphatase activity in MC 3T3-E1 cells. Dentin slice pit formation decreased with mouse osteoblast and bone marrow macrophage co-cultivation in the presence of Poly(P). Promotion of alveolar bone regeneration was observed locally in Poly(P)-treated rats. These findings suggest that Poly(P) plays a role in osteoblastic differentiation, activation, and bone mineralization. Thus, local poly(P) delivery may have a therapeutic benefit in periodontal disease.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Osteogénesis
/
Fosfatos
/
Polifosfatos
/
Pérdida de Hueso Alveolar
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Dent Res
Año:
2007
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos