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Chemoenzymatic synthesis of glutamic acid analogues: substrate specificity and synthetic applications of branched chain aminotransferase from Escherichia coli.
Xian, Mo; Alaux, Sébastien; Sagot, Emmanuelle; Gefflaut, Thierry.
Afiliación
  • Xian M; UMR 6504, Université Blaise Pascal, F-63177 Aubière Cedex, France.
J Org Chem ; 72(20): 7560-6, 2007 Sep 28.
Article en En | MEDLINE | ID: mdl-17718503
A new route to alpha-keto acids is described, based on the ozonolysis of enol acetates obtained from alpha-substituted beta-keto esters. Escherichia coli branched chain aminotransferase (BCAT) activity toward a variety of substituted 2-oxoglutaric acids was demonstrated analytically. BCAT was shown to have a broad substrate spectrum, complementary to that of aspartate aminotransferase, and to offer access to a variety of glutamic acid analogues. The usefulness of BCAT was demonstrated through the synthesis of several 3- and 4-substituted derivatives.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Glutámico / Escherichia coli / Transaminasas / Ácidos Cetoglutáricos Idioma: En Revista: J Org Chem Año: 2007 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Glutámico / Escherichia coli / Transaminasas / Ácidos Cetoglutáricos Idioma: En Revista: J Org Chem Año: 2007 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos