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Human salivary gland branching morphogenesis: morphological localization of claudins and its parallel relation with developmental stages revealed by expression of cytoskeleton and secretion markers.
Lourenço, Silvia V; Coutinho-Camillo, Cláudia M; Buim, Marcilei E C; Uyekita, Sabrina H; Soares, Fernando A.
Afiliación
  • Lourenço SV; Department of General Pathology, Dental School, University of São Paulo, São Paulo, Brazil. sloducca@usp.br
Histochem Cell Biol ; 128(4): 361-9, 2007 Oct.
Article en En | MEDLINE | ID: mdl-17687562
Development of salivary glands is a highly complex and dynamic process termed branching morphogenesis, where branched structures differentiate into mature glands. Tight junctions (TJ) are thought to play critical roles in physiological functions of tubular organs, contributing to cell polarity and preventing lateral movement of membrane proteins. Evidence demonstrated that claudins are directly involved in TJ formation and function. Using immunohistochemistry and immunofluorescence we have mapped the distribution of claudins-1, 2, 3, 4, 5, 7 and 11 and compared it with the expression of differentiation markers in human salivary glands obtained from foetuses ranging from weeks 4 to 24 of gestation. Expression of all claudins, except claudin-2 was detected in the various phases of human salivary gland development, up to fully mature salivary gland. The expression of all claudins increased according to the progression of salivary gland maturation evidenced by the classical markers-cytokeratin 14, cytokeratin low molecular weight, smooth muscle actin and human secretory component. Tight junction proteins-claudins appear to be important in the final shape and physiological functions of human salivary glands and are parallel related with markers of salivary gland differentiation.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glándulas Salivales / Citoesqueleto / Proteínas de la Membrana Límite: Humans Idioma: En Revista: Histochem Cell Biol Asunto de la revista: CITOLOGIA / HISTOCITOQUIMICA Año: 2007 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glándulas Salivales / Citoesqueleto / Proteínas de la Membrana Límite: Humans Idioma: En Revista: Histochem Cell Biol Asunto de la revista: CITOLOGIA / HISTOCITOQUIMICA Año: 2007 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania