Effect of clofibrate on the enzymes associated with oxidative stress in Wistar rat liver.
Bratisl Lek Listy
; 108(2): 56-64, 2007.
Article
en En
| MEDLINE
| ID: mdl-17685003
BACKGROUND: A diversity of endogenous and exogenous compounds have been demonstrated to cause proliferation of peroxisomes and variety of associated effects in rodents. The aim of our study was to see the effect of clofibrate on the enzymes associated with oxidative stress. METHODS: Male Wistar rats weighting 250-350 g were treated with clofibrate in a dose of 250 mg/1000 g/ 24 h for 12 days. Whole liver homogenates and subsequent subcellular fractions wrere proceeded for enzyme measurements by spectrophotometric methods. RESULTS: The activity of hydrogen producing enzymes D-aminoacid oxidase, Urate oxidase and Palmitoyl CoA oxidase was statistically significantly increased in the treated group in comparison with the control one. On the contrary to the massive increase of the Palmitoyl CoA oxidase--a marker enzyme for peroxisomal proliferation, there was only a limited increase of catalase (which inactivates hydrogen peroxide) activity. On the other hand superoxide dismutase and glutathione peroxidase activities in experimental group were down regulated in the treated group. CONCLUSION: Our results support the hypothesis that clofibrate (peroxisomal proliferators) application might produce oxidative stress in rat livers (Tab. 2, Fig. 8, Ref. 56) Full Text (Free, PDF) www.bmj.sk.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Clofibrato
/
Estrés Oxidativo
/
Hígado
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Bratisl Lek Listy
Año:
2007
Tipo del documento:
Article
Pais de publicación:
Eslovaquia