Hormonal and nutritional regulation of muscle carnitine palmitoyltransferase I gene expression in vivo.
Arch Biochem Biophys
; 465(2): 437-42, 2007 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-17673163
Transgenic mice carrying the human heart muscle carnitine palmitoyltransferase I (M-CPTI) gene fused to a CAT reporter gene were generated to study the regulation of M-CPTI gene expression. When the mice were fasted for 48 h, CAT activity and mRNA levels increased by more than 2-fold in heart and skeletal muscle, but not liver or kidney. In the diabetic transgenic mice, there was a 2- to 3-fold increase in CAT activity and CAT mRNA levels in heart and skeletal muscle which upon insulin administration reverted to that observed with the control insulin sufficient transgenic mice. Feeding a high fat diet increased CAT activity and mRNA levels by 2- to 4-fold in heart and skeletal muscle of the transgenic mice compared to the control transgenic mice on regular diet. Overall, the M-CPTI promoter was found to be necessary for the tissue-specific hormonal and dietary regulation of the gene expression.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Grasas de la Dieta
/
Carnitina O-Palmitoiltransferasa
/
Regulación Enzimológica de la Expresión Génica
/
Ayuno
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Músculo Esquelético
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Diabetes Mellitus Experimental
/
Hormonas
Límite:
Animals
Idioma:
En
Revista:
Arch Biochem Biophys
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos