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The jasmonate-induced expression of the Nicotiana tabacum leaf lectin.
Lannoo, Nausicaä; Vandenborre, Gianni; Miersch, Otto; Smagghe, Guy; Wasternack, Claus; Peumans, Willy J; Van Damme, Els J M.
Afiliación
  • Lannoo N; Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
Plant Cell Physiol ; 48(8): 1207-18, 2007 Aug.
Article en En | MEDLINE | ID: mdl-17623741
Previous experiments with tobacco (Nicotiana tabacum L. cv Samsun NN) plants revealed that jasmonic acid methyl ester (JAME) induces the expression of a cytoplasmic/nuclear lectin in leaf cells and provided the first evidence that jasmonates affect the expression of carbohydrate-binding proteins in plant cells. To corroborate the induced accumulation of relatively large amounts of a cytoplasmic/nuclear lectin, a detailed study was performed on the induction of the lectin in both intact tobacco plants and excised leaves. Experiments with different stress factors demonstrated that the lectin is exclusively induced by exogeneously applied jasmonic acid and JAME, and to a lesser extent by insect herbivory. The lectin concentration depends on leaf age and the position of the tissue in the leaf. JAME acts systemically in intact plants but very locally in excised leaves. Kinetic analyses indicated that the lectin is synthesized within 12 h exposure time to JAME, reaching a maximum after 60 h. After removal of JAME, the lectin progressively disappears from the leaf tissue. The JAME-induced accumulation of an abundant nuclear/cytoplasmic lectin is discussed in view of the possible role of this lectin in the plant.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nicotiana / Hojas de la Planta / Ciclopentanos / Lectinas de Plantas / Oxilipinas Límite: Animals Idioma: En Revista: Plant Cell Physiol Asunto de la revista: BOTANICA Año: 2007 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Japón
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nicotiana / Hojas de la Planta / Ciclopentanos / Lectinas de Plantas / Oxilipinas Límite: Animals Idioma: En Revista: Plant Cell Physiol Asunto de la revista: BOTANICA Año: 2007 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Japón