Analysis of a challenging subset of World Anti-Doping Agency-banned steroids and antiestrogens by LC-MS-MS.
J Anal Toxicol
; 31(3): 125-31, 2007 Apr.
Article
en En
| MEDLINE
| ID: mdl-17579958
A qualitative liquid chromatography-tandem mass spectrometry method for the analysis of 22 sporting federation-banned anabolic agents (or their metabolite markers) and anti-estrogens in urine that are refractory to analysis by gas chromatography-mass spectrometry is presented. In addition, a quantitative method built around World Anti-Doping Agency (WADA) guidelines for the confirmatory analysis of 19-norandrosterone, the primary metabolite of nandrolone with a WADA-specified minimum required performance limit of 1 ng/mL, is included. Hydrolysis of glucuronide conjugates, liquid-liquid extraction, no clean-up derivatization with Girard's Reagent P, and analysis by quadrupole-time-of-flight mass spectrometry provide sensitivity and selectivity well beyond that required by the WADA.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Detección de Abuso de Sustancias
/
Cromatografía Líquida de Alta Presión
/
Moduladores de los Receptores de Estrógeno
/
Espectrometría de Masa por Ionización de Electrospray
/
Doping en los Deportes
/
Estranos
/
Espectrometría de Masas en Tándem
/
Anabolizantes
Tipo de estudio:
Diagnostic_studies
/
Guideline
/
Qualitative_research
Límite:
Humans
Idioma:
En
Revista:
J Anal Toxicol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Reino Unido