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Novel MEN1 germline mutations in Brazilian families with multiple endocrine neoplasia type 1.
Toledo, Rodrigo A; Lourenço, Delmar M; Coutinho, Flavia L; Quedas, Elisangela; Mackowiack, Ivone; Machado, Marcel C C; Montenegro, Fabio; Cunha-Neto, Malebranche B C; Liberman, Bernardo; Pereira, Maria A A; Correa, Pedro H S; Toledo, Sergio P A.
Afiliación
  • Toledo RA; Unidade de Endocrinologia Genética LIM-25, Endocrinologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo, Brazil.
Clin Endocrinol (Oxf) ; 67(3): 377-84, 2007 Sep.
Article en En | MEDLINE | ID: mdl-17555499
OBJECTIVE: To characterize clinical features and identify MEN1 germline mutations in Brazilian families with multiple endocrine neoplasia type 1 (MEN1). Settings Non-profit academic centre. PATIENTS: Fourteen Brazilian families with MEN1 and 141 at-risk relatives. RESULTS: We identified 12 different MEN1 disease-causing mutations, seven of them previously unreported: 308delC; 375del21; 549A>T (I147F); 1243delA; 1348T>G (L413R); 1351T>C (L414P) and 1523G>T (W471C). Families with the recurrent mutations 360delTCTA and L413R were shown to be unrelated by mitochondrial-DNA and Y-chromosome haplotype analyses. Most of the MEN1 single point mutations involved evolutionarily conserved residues, whereas most of the deletion/frameshift changes occurred in GC-rich repetitive regions. Genetic screening of 141 at-risk family members identified 38 MEN1 mutation carriers, 37 (97.4%) of whom had at least one major MEN1-related tumour upon clinical investigation. CONCLUSIONS: High frequencies of MEN1 gene mutations were detected in Brazilian families with MEN1, including seven new genetic mutations that are predicted to cause inactivation of the MEN1 tumour suppressor gene. Our data underscore the need to implement a systematic MEN1 screening programme in Brazil.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Proto-Oncogénicas / Mutación de Línea Germinal / Neoplasia Endocrina Múltiple Tipo 1 Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged País/Región como asunto: America do sul / Brasil Idioma: En Revista: Clin Endocrinol (Oxf) Año: 2007 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Proto-Oncogénicas / Mutación de Línea Germinal / Neoplasia Endocrina Múltiple Tipo 1 Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged País/Región como asunto: America do sul / Brasil Idioma: En Revista: Clin Endocrinol (Oxf) Año: 2007 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido