The antiglucocorticoid RU486 downregulates the expression of interleukin-2 receptors in normal human lymphocytes.
J Steroid Biochem Mol Biol
; 39(6): 929-35, 1991 Dec.
Article
en En
| MEDLINE
| ID: mdl-1751392
ABSTRACT
PIP: The effects of RU-486, which has anti-glucocorticoid properties, on expression of the interleukin-2 receptors (IL-2R) in human lymphocytes were examined in terms of its dose effect on 2 types of binding sites, and its suppression of beta-chain IL-2R messenger RNA production. Human peripheral lymphocytes stimulated with phytohemagglutinin (PHA) were incubated with the synthetic glucocorticoid dexamethasone and with RU-486 at various doses. Synthesis of 2 types of IL-2R was measured by immunofluorescence assay of the alpha chain. RU-486 inhibits in a dose- dependent fashion the expression of both classes of IL-2R, acting like an agonist. Maximal suppression was seen at a concentration of 10 mcM for the low affinity binding site, and at 1 mcM for the high affinity rate. Messenger RNA was determined by extracting RNA, fractioning it by electrophoresis on agarose gel, and hybridizing with cDNA probes inserted with a plasmid. RU-486 significantly lowered the accumulation of beta-chain IL-2R mRNA transcripts. Thus RU-486 at pharmacological concentrations acts like a glucocorticoid agonist with immunosuppressive effects.
Palabras clave
Adrenal Cortex Hormones; Biology; Clinical Research; Cytologic Effects; Developed Countries; Dexamethasone Acetate; Endocrine System; Europe; Greece; Hormone Antagonists; Hormone Receptors; Hormones; Immunity; Immunologic Factors; In Vitro; Mediterranean Countries; Membrane Proteins; Physiology; Research Methodology; Research Report; Ru-486; Southern Europe
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Activación de Linfocitos
/
Receptores de Interleucina-2
/
Regulación hacia Abajo
/
Mifepristona
Límite:
Humans
Idioma:
En
Revista:
J Steroid Biochem Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
1991
Tipo del documento:
Article
País de afiliación:
Grecia
Pais de publicación:
Reino Unido