Genomewide identification of nuclear matrix attachment regions: an analysis of methods.

Linnemann, A K; Platts, A E; Doggett, N; Gluch, A; Bode, J; Krawetz, S A

Linnemann, A K; Platts, A E; Doggett, N; Gluch, A; Bode, J; Krawetz, S A.

Afiliación

Linnemann AK; Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, USA.

Biochem Soc Trans ; 35(Pt 3): 612-7, 2007 Jun.

Article en En | MEDLINE | ID: mdl-17511663

RESUMEN

High-throughput technologies now afford the opportunity to directly determine the distribution of MARs (matrix attachment regions) throughout a genome. The utility of cosmid and oligonucleotide platforms to identify human chromosome 16 MARs from preparations that employed LIS (lithium di-iodosalicylic acid) and NaCl extraction protocols was examined. The effectiveness of the platforms was then evaluated by Q-PCR (quantitative real-time PCR). Analysis revealed that caution must be exercised, since the representation of non-coding regions varies among platforms. Nevertheless, several interesting trends were revealed. We expect that these technologies will prove useful in systems approaches directed towards defining the role of MARs in various cell types and cellular processes.

Asunto(s)

Matriz Nuclear/genética; Matriz Nuclear/metabolismo; Sitios de Unión/genética; Cromosomas Humanos Par 16/genética; Cromosomas Humanos Par 16/metabolismo; Genoma Humano; Humanos; Análisis de Secuencia por Matrices de Oligonucleótidos

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Matriz Nuclear Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Biochem Soc Trans Año: 2007 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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