[Research into methylation of p16 gene in the patients suffering from coal-burnt arsenism in Xingren County Guizhou Province].
Zhonghua Yu Fang Yi Xue Za Zhi
; 41(1): 29-32, 2007 Jan.
Article
en Zh
| MEDLINE
| ID: mdl-17484207
OBJECTIVE: To probe into the situation and significance of p16 gene CPG island methylation in patients with arseniasis caused by coal-burning pollution. METHODS: DNA was extracted using the Phenol-Chloroform method from leukocytes of 51 patients suffered from coal-burnt arsenism and 52 healthy volunteers. The quantity of the DNA was determined by UV spectrophotometry. Target DNA was denatured by NaOH, then the single strand DNA was modified by sodium bisulfite, converting all unmethylated (but not the methylated) cytosines to uracil. Subsequently a nested amplification with primers specific for methylated versus unmethylated DNA was performed, and PCR products were detected by gel electrophoresis. RESULTS: Hypermethylation of the p16 CPG island was presented in 94.1% of the patients suffering from coal-burnt arsenism and in 73.1% of the healthy volunteers. There was statistical difference (P < 0.05) between them. CONCLUSIONS: Methylation of p16 gene CPG island should have important pertinence in the metabolism of coal-burnt arsenism.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carbón Mineral
/
Metilación de ADN
/
Genes p16
/
Intoxicación por Arsénico
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Yu Fang Yi Xue Za Zhi
Año:
2007
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China