A quantitative assessment of the carbonic anhydrase activity in photosystem II.

McConnell, I L; Badger, M R; Wydrzynski, T; Hillier, W

McConnell, I L; Badger, M R; Wydrzynski, T; Hillier, W.

Afiliación

McConnell IL; Research School of Biological Sciences, The Australian National University, Canberra, ACT 0200, Australia. Iain.Mcconnell@anu.edu.au

Biochim Biophys Acta ; 1767(6): 639-47, 2007 Jun.

Article en En | MEDLINE | ID: mdl-17467655

RESUMEN

Using a carbonic anhydrase assay based on membrane inlet mass spectrometry (MIMS), we have extended our earlier investigations of Photosystem II (PSII)-associated carbonic anhydrase activity in spinach PSII preparations (W. Hillier, I. McConnell, M. R. Badger, A. Boussac, V.V. Klimov G. C. Dismukes, T. Wydrzynski Biochemistry 2006, 45:2094). The relationship between the carbonic anhydrase activity and O(2) evolution has been evaluated in terms of the effects of metal ion addition, preparation type, light, and response to specific inhibitors. The results indicate that the PSII-associated carbonic anhydrase activity is variable and appears not to be associated specifically with the oxygen evolving activity nor the 33 kDa extrinsic manganese stabilising protein.

Asunto(s)

Anhidrasas Carbónicas/análisis; Complejo de Proteína del Fotosistema II/química; Complejo de Proteína del Fotosistema II/metabolismo; Bicarbonatos/metabolismo; Dióxido de Carbono/metabolismo; Anhidrasas Carbónicas/metabolismo; Cinética; Oxígeno/metabolismo; Isótopos de Oxígeno; Spinacia oleracea/metabolismo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anhidrasas Carbónicas / Complejo de Proteína del Fotosistema II Idioma: En Revista: Biochim Biophys Acta Año: 2007 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos

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