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Arg-gingipain A DNA vaccine prevents alveolar bone loss in mice.
Miyachi, K; Ishihara, K; Kimizuka, R; Okuda, K.
Afiliación
  • Miyachi K; Department of Microbiology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba, 261-8502, Japan.
J Dent Res ; 86(5): 446-50, 2007 May.
Article en En | MEDLINE | ID: mdl-17452566
One major pathogenic factor of Porphyromonas gingivalis is Arg-gingipain (Rgp), an arginine-specific cysteine proteinase. To clarify the effect of rgpA DNA vaccine, we immunized BALB/c mice via the abdomen with a Gene Gun or via the nasal cavity weekly for 6 weeks. After immunization, the mice were challenged orally with P. gingivalis. Immunization elicited IgG responses against P. gingivalis in both groups. Nasal immunization also induced sIgA against P. gingivalis, although Gene Gun immunization did not. Reduction of alveolar bone loss was observed in both groups at 42 days following initial infection. This effect was more pronounced in the intranasal immunization group than in the Gene Gun group. The results of this study suggest that immunization with rgpA DNA vaccine via the nasal cavity is an effective method for preventing alveolar bone loss incurred by infection with P. gingivalis.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cisteína Endopeptidasas / Vacunas Bacterianas / Pérdida de Hueso Alveolar / Porphyromonas gingivalis / Adhesinas Bacterianas / Vacunas de ADN Límite: Animals Idioma: En Revista: J Dent Res Año: 2007 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cisteína Endopeptidasas / Vacunas Bacterianas / Pérdida de Hueso Alveolar / Porphyromonas gingivalis / Adhesinas Bacterianas / Vacunas de ADN Límite: Animals Idioma: En Revista: J Dent Res Año: 2007 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos