The acinar location of the sodium-independent and the sodium-dependent component of taurocholate uptake. A histoautoradiographic study of rat liver.
J Hepatol
; 13(2): 169-78, 1991 Sep.
Article
en En
| MEDLINE
| ID: mdl-1744421
The acinar location of tritium-labelled taurocholate taken up from sodium-containing and sodium-free perfusion media in isolated perfused rat liver was made visible by means of histoautoradiography on cryoslices at low, medium and high bile salt concentrations. In antegrade perfusion studies, in the presence of sodium, with taurocholate concentrations of 1 and 20 microM, respectively, the silver grain label was mainly restricted to acinar zones 1. At an 80 microM concentration, zones 2 and 3 were also labelled but with decreasing intensities towards the terminal hepatic venules. At 120 microM taurocholate, all acinar zones were nearly equally labelled. In the absence of sodium, antegrade liver perfusions with 1, 20 and 120 microM taurocholate resulted in an almost homogenous labelling of all acinar zones and retrograde perfusions in a silver grain density slightly decreasing towards the terminal portal venules. The results indicate that hepatocytes of all acinar zones are capable of taking up taurocholate by both a sodium-dependent and a sodium-independent pathway. The contribution of the sodium-independent uptake to the overall uptake of taurocholate increases with increasing zonal recruitment at higher concentrations. The sodium-dependent uptake, however, is always dominant.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sodio
/
Ácido Taurocólico
/
Hígado
Límite:
Animals
Idioma:
En
Revista:
J Hepatol
Asunto de la revista:
GASTROENTEROLOGIA
Año:
1991
Tipo del documento:
Article
Pais de publicación:
Países Bajos