Hybrid titania-aminosilane platforms evaluated with human mesenchymal stem cells.
J Biomed Mater Res B Appl Biomater
; 83(1): 232-9, 2007 Oct.
Article
en En
| MEDLINE
| ID: mdl-17385221
The properties of hybrid aminopropyltriethoxysilane-tetraisopropylorthotitanate (APTS-TIPT) platforms prepared by a sol-gel route have been explored, and their biocompatibility was assayed after culture of human mesenchymal stem cells (hMSCs). The organic content of this material was observed to be preferably surface-oriented as indicated by microanalytical techniques. Furthermore, the surface showed characteristic amino-silane bands when explored by Raman spectroscopy as well as indications of silane and titanate condensation. Surface activity of the amino groups was probed by ultraviolet-visible spectroscopy imine derivatization and chemical force spectroscopy, showing a pH-dependent surface charge-induced potential. hMSCs cultured onto these surfaces showed relevant differences with respect to their behavior on gelatin-coated glass plates. Even if with a lower proliferative rate than controls, the cells develop long cytosolic prolongations in osteogenic differentiation medium, thus, supporting the idea of an APTS-TIPT stimulated process.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Silanos
/
Titanio
/
Materiales Biocompatibles
/
Células Madre Mesenquimatosas
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
J Biomed Mater Res B Appl Biomater
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos