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Bio-electrosprays: a novel electrified jetting methodology for the safe handling and deployment of primary living organisms.
Odenwälder, Patrick K; Irvine, Scott; McEwan, Jean R; Jayasinghe, Suwan N.
Afiliación
  • Odenwälder PK; Department of Mechanical Engineering, University College London, Torrington Place, London, UK.
Biotechnol J ; 2(5): 622-30, 2007 May.
Article en En | MEDLINE | ID: mdl-17373645
Electrohydrodynamic jetting (EHDJ) which is also known as electrosprays (ES) has recently been elucidated as a unique electrified biotechnique for the safe handling and deployment of living organisms. This high intensity electric field driven jetting methodology is now referred to as "bioelectrosprays" (BES). Previously these charged jets have only been shown to jet-process immortalized cells which have undergone expected cellular behavior when compared with control cells. In this paper we demonstrate the ability to jet process primary living organisms in the stable conejetting mode. Finally the viability of the bio-electrosprayed living organisms has been assessed employing a flow cytometry approach which forms the discussion in this paper. Our findings further establish BES as a competing biotechnique, which could be employed for the deposition of primary living organisms according to a predetermined active cellular architecture. One day this could be used for the fabrication of viable tissues and organs for repair or replacement. These advanced studies carried out on BES have direct widespread applications ranging from developmental biology to regenerative and therapeutic medicine, which are a few amongst several other areas of study within the life sciences.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Manejo de Especímenes / Biotecnología / Técnicas de Cultivo de Célula / Microfluídica / Técnicas de Cultivo de Tejidos / Electroquímica Tipo de estudio: Health_technology_assessment Idioma: En Revista: Biotechnol J Asunto de la revista: BIOTECNOLOGIA Año: 2007 Tipo del documento: Article Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Manejo de Especímenes / Biotecnología / Técnicas de Cultivo de Célula / Microfluídica / Técnicas de Cultivo de Tejidos / Electroquímica Tipo de estudio: Health_technology_assessment Idioma: En Revista: Biotechnol J Asunto de la revista: BIOTECNOLOGIA Año: 2007 Tipo del documento: Article Pais de publicación: Alemania