Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes.
Bioresour Technol
; 99(3): 463-71, 2008 Feb.
Article
en En
| MEDLINE
| ID: mdl-17369037
The white rot fungus Irpex lacteus is able to decolorize such synthetic dyes as Reactive Orange 16 and Remazol Brilliant Blue R. Here, we demonstrate that this type of dye decolorization is mainly related to a laccase-like enzyme activity associated with fungal mycelium. In its bound form, the enzyme detected showed a pH optimum of 3.0 for the oxidation of ABTS, DMP and guaiacol, and a pH of 7.0 for syringaldazine. The highest enzymatic activity was obtained with ABTS as substrate. Enzyme activity was fully inhibited with 50mM NaN(3). Depending on the chemical structure of dyes, redox mediators had a positive effect on the dye decolorization by fungal mycelium. Enzyme isolated from fungal mycelium was able to decolorize synthetic dyes in vitro.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Basidiomycota
/
Micelio
/
Lacasa
/
Colorantes
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Bioresour Technol
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2008
Tipo del documento:
Article
País de afiliación:
República Checa
Pais de publicación:
Reino Unido