The investigation of Chlamydophila pneumoniae in patients with multiple sclerosis.
Int J Neurosci
; 117(3): 409-15, 2007 Mar.
Article
en En
| MEDLINE
| ID: mdl-17365124
Totally 32 cerebrospinal fluid samples from Multiple sclerosis (MS) patients were collected. DNA was extracted by High Pure PCR Template Preparation Kit. Two genomic segments, outer membrane protein genes ompA and omp9, were targeted for the detection of C. pneumoniae DNA in the samples by PCR tests. To detect ompA, a nested-PCR assay was designed, whereas for omp9, a PCR-Enyzme immunoassay (PCR-EIA) depending on streptavidin-biotin capture and dig detection of the PCR products was performed. C. pneumoniae DNA was not detected by each assays in patient samples.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Chlamydophila pneumoniae
/
Infecciones por Chlamydophila
/
Esclerosis Múltiple
Límite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Int J Neurosci
Año:
2007
Tipo del documento:
Article
País de afiliación:
Turquía
Pais de publicación:
Reino Unido