[Studies of the primary effects of chronic clinostatting of embryonal mice stem cells in vitro].
Aviakosm Ekolog Med
; 40(5): 34-7, 2006.
Article
en Ru
| MEDLINE
| ID: mdl-17357625
Effects of randomized gravity vector (clinostatting) on embryonal stem cells (ESC) of mice were evaluated in vitro with respect to proliferation, proliferative potential, and differentiability. Colony formation remained normal up to hour 72 of clinostatting; however, further exposure led to fusion of the ESC colonies. No reliable shifts in the proliferative activity were found, whereas morphometric analysis showed different dynamics of the ESC colonies size in specific periods of the experiment comparing with the control. Evaluation of the ESC proliferative potential after the experiment revealed a trend upward in the number of colonies when compared with the dynamic and static controls. However, the number of resulted EBs in the control tended upward contrary to EBs formed under the conditions of clinostatting and continuous agitation pointing to the importance of local medium conditioning at the beginning of ESC differentiation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rotación
/
Células Madre Embrionarias
Límite:
Animals
Idioma:
Ru
Revista:
Aviakosm Ekolog Med
Asunto de la revista:
MEDICINA AEROESPACIAL
/
SAUDE AMBIENTAL
Año:
2006
Tipo del documento:
Article
Pais de publicación:
Rusia