Homologous regulation of estrogen receptor subtypes in goldfish (Carassius auratus).
Mol Reprod Dev
; 74(9): 1105-12, 2007 Sep.
Article
en En
| MEDLINE
| ID: mdl-17342729
While considerable information is available on the physiological effects of estrogen, much less is known about the regulation of estrogen receptor (ER) subtypes, particularly in non-mammalian vertebrates. Using goldfish as primary experimental model, we investigated sex- and tissue-specific homologous regulation of ER subtypes (ERalpha, ERbetaI, and ERbetaII) by estradiol in vivo, in the liver and gonads. Treatment with estradiol, significantly upregulated transcript levels for all three types of ERs (ERalpha, ERbetaI, and ERbetaII) in the goldfish ovary and testis. In the goldfish liver, treatment with estradiol significantly increased ERalpha, ERbetaI transcript levels without affecting ERbetaII. In all cases increased ER transcript level was correlated with increased ER protein level determined by Western blot analysis, although we are not able to distinguish between ER subtypes. The results provide strong support for the hypothesis that homologous regulation of ERs is tissue- and gender-specific, and may be a mechanism for estrogen-mediated regulation of reproduction in goldfish.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Reproducción
/
Carpa Dorada
/
Receptores de Estrógenos
/
Estradiol
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Reprod Dev
Asunto de la revista:
BIOLOGIA MOLECULAR
/
MEDICINA REPRODUTIVA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
Estados Unidos