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Noncovalent protein transduction in plant cells by macropinocytosis.
Chang, Microsugar; Chou, Jyh-Ching; Chen, Chung-Pin; Liu, Betty Revon; Lee, Han-Jung.
Afiliación
  • Chang M; Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
  • Chou JC; Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
  • Chen CP; Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
  • Liu BR; Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
  • Lee HJ; Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
New Phytol ; 174(1): 46-56, 2007.
Article en En | MEDLINE | ID: mdl-17335496
* Protein delivery across cellular membranes or compartments is primarily limited by low biomembrane permeability. * Many protein transduction domains (PTDs) have previously been generated, and covalently cross-linked with cargoes for cellular internalization. * An arginine-rich intracellular delivery (AID) peptide could rapidly deliver fluorescent proteins or beta-galactosidase enzyme into plant and animal cells in a noncovalent fashion. The possible mechanism of this noncovalent protein transduction (NPT) may involve macropinocytosis. * The NPT via a nontoxic AID peptide provides a powerful tool characterized by its simplicity and quickness to have active proteins function in living cells in vivo. This should be of broad utility for functional enzyme assays and protein therapies in both plant biology research as well as biomedical applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Pinocitosis / Plantas / Proteínas Portadoras / Transporte de Proteínas Límite: Humans Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2007 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Pinocitosis / Plantas / Proteínas Portadoras / Transporte de Proteínas Límite: Humans Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2007 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido